Traditional antibody selection methods have their limits.
Antibody selection is a critical step in developing high-performance diagnostic assays, yet traditional methods provide limited insight into how antibodies will perform under final assay conditions. Because selection is typically based on equilibrium measurements (ELISA), high-performing candidates may be missed and suboptimal antibodies may be taken forward into assay development, which can lead to re-selection and cost months of development time.
First Time Right, select the right binders from the start.
We call our antibody selection platform First Time Right because it guarantees the identification of the best antibody, or antibody pair, from the very first screen.
This platform combines Kimialys’ K-One surface chemistry with the multiplexing capabilities of SPRi to characterize antibody candidates through real-time association and dissociation measurements. We also assess cross-reactivity and the impact of buffers and complex matrices on antibody performance.
Streamline antibody selection, save time and reduce costs.
We typically select your antibodies within 1 to 2 weeks, using fewer antibodies and assay materials. One example is sandwich LFA development, where an initial ELISA-based selection often has to be followed by combinatorial testing of multiple capture and detection antibody pairs, rapidly increasing the number of tests, materials, and iterations required (a process that can take several weeks). By delegating antibody selection to Kimialys, you can identify better candidates faster, reduce costs, and avoid labor-intensive workflows.
Reliable antibody selection
Kinetic characterization, buffer optimization, cross-reactivity assessment, and binning assays help ensure that the most promising candidates are identified early.
Fast and efficient
SPRi multiplexing enables parallel screening of multiple antibody candidates. Antibody selection typically takes 1 to 2 weeks.
Minimal reagent usage
Our approach requires only small amounts of antibodies and assay materials.
Broad compatibility
Selected antibodies can be transferred to multiple surface formats (e.g., nanoparticles)
Compatible with common capture systems (e.g., streptavidin)
-SPRi-
Surface Plasmon Resonance imaging (SPRi) is an advanced optical biosensing technology that enables real-time, label-free analysis of molecular interactions. It allows for the simultaneous screening of multiple antibodies on a chip, providing kinetic and affinity data (kon, koff, KD). This multiplexing capability of SPRi not only saves time but also greatly minimizes the consumption of antibodies, target biomarkers, and other assay materials.
-K-One-
K-One is Kimialys’ proprietary surface chemistry for gold surfaces. It enables precise control of surface interactions, delivering reliable data with minimal background noise.
In antibody selection projects, we functionalize sensor chips with K-One and optimize the immobilization conditions for each antibody individually, so that binding kinetics are assessed under that antibody’s optimal conditions. Once the best candidate or antibody pair has been identified, both the selected binders and the optimized immobilization conditions can be readily transferred to our gold nanoparticles. This creates a direct bridge between antibody screening and assay development.
For LFA projects, for example, once the optimal antibody or antibody pair has been identified, we can support proof-of-concept development by leveraging our expertise in bioconjugation.
-First Time RIght-
Robust kinetic analysis: affinity (KD), association rate (kon), and dissociation rate (koff).
Optimization of immobilization conditions for each antibody tested on-chip, ensuring optimal orientation and minimizing measurement bias.
Cross-reactivity assessment in both buffer and real biological samples, including urine, plasma, serum, and saliva.
Binning analysis, essential for sandwich formats, to determine which antibody performs best as the capture antibody and which as the detection antibody within each compatible pair.
This unique combination of methodology and technology makes Kimialys a strong partner for antibody screening and assay development. While LFA is a key application area, our platform also supports other colorimetric assays and immunoprecipitation workflows (e.g., IP-MS).
You’ll first connect with our scientific sales team to discuss your needs and project goals.
You’ll meet our technical team to dive deep into your application and challenges. Based on these discussions, we build a detailed work plan and list all required materials.
Once we agree on scope, timing, and cost, we move straight to practical implementation.
One week before work begins, we hold a comprehensive kick-off meeting where all details are finalized and final questions are answered. This is the moment to meet the person in charge of the project at Kimialys, who will be your contact throughout the entire project.
From there, you stay connected with our scientists at every step, keeping you fully informed on progress.
We deliver a comprehensive presentation and final report summarizing all results and key findings. By the end of the project, you know which antibodies to move forward with for further development.
When relevant, we can also support further development by leveraging K-One on gold nanoparticles and our in-house bioconjugation expertise (e.g., for LFA proof-of-concept development, colorimetric assays, IP-MS).
Curious about how our FTR approach can guarantee your needs are met?
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Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms
Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent
For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.
We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.
Gold-based sensor types:
For common SPR and SPRi platforms: Biacore, Horiba...
Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...
For chip-based diagnostic applications: electrochemical biosensors, QCM...
Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.
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