Complete characterization through precise kinetics and reliable results.
We partner with leading biotech, pharmaceutical, and AI drug discovery companies, as well as CROs and CDMOs, to deliver advanced biophysical analysis services, from molecular interaction screening to in-depth kinetic characterization.
K-One: unlocking the full potential of SPR systems.
Powered by our proprietary K-One surface functionalization chemistry, we push the boundaries of SPR, enabling applications previously out of reach with conventional sensor chips. Our coating enhances SPR performance through precise ligand immobilization, optimized orientation, controlled surface density for accurate 1:1 Langmuir fits, and protection of the surface from non-specific interactions, delivering exceptionally clean data with minimal background noise.
Tailored assay design is key to robust data.
At Kimialys, each assay is designed around the specific application and molecules involved, with its own dedicated protocol. From ligand immobilization strategy to instrument calibration, injection sequences, and regeneration workflows, every parameter is carefully tuned to deliver robust, reproducible assays suited to complex biological systems.
Mastering complex systems
We enable the most challenging SPR assays by optimizing sensor chips with our K-One chemistry, whether the challenge lies in improving signal-to-noise ratio, immobilizing complex biomolecules, reducing avidity effects, or accurately characterizing high-affinity AI-designed molecules.
Science without noise
With K‑One, the chip surface is effectively protected from non-specific interactions, minimizing background noise and delivering consistently clean, reliable sensorgrams, even in complex samples.
High reproducibility
Stable and controlled ligand immobilization enables repeated injection and regeneration cycles while maintaining full ligand activity on the surface, even under stringent conditions. This stability ensures repeatable results across complex samples such as plasma, serum, or supernatants.
Reliable 1:1 Langmuir fit
Our ability to adjust ligand density and ensure homogeneous surface distribution allows working under affinity conditions by minimizing avidity effects or mass transport while avoiding recapture. We can also work under avidity conditions when the project requires it.
Flexible immobilization
K-One surface chemistry enables direct ligand immobilization without intermediates, while remaining compatible with capture systems such as streptavidin, protein A/G, anti-Fc and anti-Fab antibodies.
Analytical SPR services
Binding interaction screening
Kinetics characterization:
Epitope binning and cross‑reactivity assessment
Key benefits
Enabling complex systems
Science without background noise
Tailored assay development
Working under true affinity conditions
Stable immobilization and high reproducibility
Molecular entities we characterize
Antibodies:
Proteins:
Oligonucleotides:
Other entities:
AI-designed biomolecules
In the era of AI-driven biomolecule design, where antibodies and engineered proteins are increasingly generated in silico with predicted KD, kon, and koff values, reliable experimental validation under application-relevant conditions is critical. K-One enables high-fidelity kinetic characterization of highly affine and structurally optimized molecules in conditions that closely reflect their intended use, generating robust datasets that support sequence refinement and iterative model improvement.
You’ll first connect with our scientific sales team to discuss your needs and project goals.
You’ll meet our technical team to dive deep into your application and challenges. Based on these discussions, we build a detailed work plan and list all required materials.
Once we agree on scope, timing, and cost, we move straight to practical implementation.
One week before work begins, we hold a comprehensive kick-off meeting where all details are finalized and final questions are answered. This is the moment to meet the person in charge of the project at Kimialys, who will be your contact throughout the entire project.
From there, you stay connected with our analyst at every step, keeping you informed on progress via regular video calls, where intermediate results are explained and discussed.
We deliver a comprehensive presentation and final report, with all methods, results, and data interpretations clearly explained, allowing you to confidently interpret the conclusions. We also provide the raw data.
Curious about how our Surface Plasmon Resonance expertise can resonate with your current needs?
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Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms
Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent
For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.
We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.
Gold-based sensor types:
For common SPR and SPRi platforms: Biacore, Horiba...
Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...
For chip-based diagnostic applications: electrochemical biosensors, QCM...
Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.
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