Kinetic-driven antibody selection delivers guaranteed reliability and enhanced precision.
Antibody screening is a critical step in developing high-performance diagnostic assays, yet traditional methods lack reliability, as selection is typically performed at equilibrium (e.g. ELISA or combinatorial LFA screening).
At Kimialys, we combine the K-One sensor chip with the multiplexing power of SPRi to characterize antibody candidates based on real-time association and dissociation rates. That’s why we call our approach FirstTimeRight, as we identify the best antibody — or pair — from the very first screen.
Our SPRi-based screening offers more than just affinity ranking:
- Cross-reactivity validation in real biological samples
- Epitope binning for precise capture/detection assignment
- Optimization of immobilization conditions and buffers
- Reduced consumption of antibodies and target materials
Solid-phase immunoassays
LFA / i-PCR
Liquid-phase immunoassays
Turbidimetry / Nephelometry
Target isolation
Immunoprecipitation
Fast antibody selection
We identify the optimal antibodies for your application in just one week — compared to several weeks with conventional approaches.
Unmatched precision
We optimize buffer and immobilization conditions for each antibody and assess cross-reactivity through epitope binning — ensuring no candidates are overlooked.
Minimal reagent usage
Our method requires only small amounts of antibodies and target, making the selection process highly resource-efficient.
Compatible with all detection labels
Our selection process works seamlessly with all label types — such as gold nanoparticles, latex beads, magnetic particles.
Traditionally, antibody selection relies on trial and error, requiring assay developers to go through a labor-intensive screening process. This often involves ELISA or functional assays to evaluate multiple candidates. However, this approach lacks real-time kinetic insights, is time-consuming, and risks missing the most effective antibodies due to suboptimal immobilization or conjugation.
At Kimialys, we leverage the K-One sensor chip together with SPRi's multiplexing capabilities to analyze antibody candidates. That’s the essence of our FirstTimeRight approach: selecting the optimal antibody — or pair — from the very first screen.
Surface Plasmon Resonance imaging (SPRi) is an advanced optical biosensing technology that enables real-time, label-free analysis of molecular interactions. It allows for the simultaneous screening of multiple antibodies on a chip, providing kinetic and affinity data. This multiplexing capability of SPRi significantly saves time but also minimizes the consumption of antibodies and target material.
SPRi also delivers additional analytical value through buffer optimization and validated cross-reactivity testing in real biological samples — such as urine, plasma, serum, or saliva.
Finally, binning analysis — essential for sandwich formats like LFA, iPCR, or ELISA — precisely determines which antibody functions best as capture and which as detection within each compatible pair.
This unique integration of method and technology positions Kimialys at the forefront of antibody screening for diagnostic assays.
You’ll first connect with our sales team (Nicolas, Adrien or Cyril) to discuss your needs and project goals.
You’ll meet our technical team to dive deep into your application and challenges. Based on these discussions, we build a detailed work plan and list all required materials. Once we agree on scope, timing, and cost, we move straight to practical implementation
One week before work begins, we hold a comprehensive kick-off meeting where all details are finalized and final questions are answered. This is the moment to meet the person in charge of the project at Kimialys, who will be your contact throughout the entire project. From there, you stay connected with our experimentalists at every step, keeping you fully informed on progress.
We deliver a comprehensive final report with all results and findings. Based on our initial agreement, you receive your optimized K-One biochips with buffers and detailed protocols — no chocolate included!
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Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms
Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent
For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.
We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.
Gold-based sensor types:
For common SPR and SPRi platforms: Biacore, Horiba...
Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...
For chip-based diagnostic applications: electrochemical biosensors, QCM...
Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.
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