Discover our premium-quality gold bioconjugates and redefine your standards.
Leveraging Kimialys’ proprietary surface chemistry, K-One, we develop, manufacture, and supply high-performance gold bioconjugates optimized to your biomolecules and applications. K-One provides full control over surface interactions and protects against non-specific binding, ensuring sensitive, reliable, and reproducible results, without trading one parameter off against another.
Our bioconjugates selectively capture target molecules to enable detection assays as well as extraction for enrichment or depletion workflows, supporting diagnostic and biopharma companies across a wide range of applications including analytical characterization, immunogenicity testing, molecular diagnostics, and point-of-care testing.
When conventional nanoparticles reach their limits.
For many use cases, conventional gold, magnetic, latex, or luminescent nanoparticles perform perfectly well. But when targets are present at very low concentrations, or when matrices become too complex, such approaches share common limitations in terms of sensitivity, reproducibility, and stability—often improving one parameter at the expense of the others.
Gold nanoparticles are widely employed as bioconjugates prepared by passive adsorption for LFA or colorimetric assays, valued for their ease of use and their strong optical response in the visible range.
Magnetic beads have long been the industry standard for targeted capture and extraction, thanks to their ease of use and compatibility with automated platforms.
Latex beads are widely used in turbidimetry and nephelometry, valued for their ease of use and strong light-scattering properties. They are also available in multiple colors for straightforward multiplexing for LFA applications and in fluorescent variants that deliver higher sensitivity.
Luminescent nanoparticles, such as quantum dots (QDs) and up-conversion nanoparticles (UCNPs), are the latest generation of nanoparticles. More expensive than conventional alternatives, these nanoparticles are valued for their very high sensitivity and narrow emission spectra, which enable advanced multiplexing.
K-One gold nanoparticles are designed for challenging assays where targets are present at very low concentrations in complex matrices, where achieving high sensitivity without compromising on non-specific binding, stability, or reproducibility is essential.
Ultimately, K-One lowers assay costs by reducing ligand consumption (up to 30×), as controlled orientation and density avoid the need to saturate the surface.
| Criteria | Standard gold NPs | Magnetic | Latex (colori) | Latex (fluo) | Luminescent (QDs, UCNPs) | K-one gold NPs |
|---|---|---|---|---|---|---|
| Analytical sensitivity |  | | | | |  |
| Selectivity (no NSB) | | | | | | |
| Cost savings | | | | | | |
| Automation compatibility | | | | | | |
| Multiplex color capability |  - | - |  | | | - |
Table: Performance of K-One gold nanoparticles compared to standard nanoparticles and beads.
Point-of-care testing
Lateral Flow Assays (LFA)
LAMP-LFA
Nanoparticle-based signal amplification
Bioanalysis
Immunoprecipitation (e.g. IP–MS, ADC–DAR, IP–Western)
Immunodepletion for interfering molecules (e.g. HSA)
Immunogenicity testing (e.g. depletion of ADA)
Molecular diagnostics
PCR workflows (immuno-PCR, qPCR, dPCR, multiplex PCR)
Isothermal amplification (LAMP)
Analytical characterization
Turbidimetry / Nephelometry
Colorimetry
High sensitivity
Increase your analytical sensitivity by up to 10× compared to conventional bioconjugates.
High selectivity
Drastically reduce your non‑specific binding and false positive results thanks to K‑One surface protection.
High reproducibility & stability
Obtain accurate and reproducible results across complex samples, including blood, plasma, saliva, urine and milk.
Cost-effectiveness
Save up to 30x in ligand consumption, by working with the optimal ligand density for your assay.
Compatible with capture systems
Keep the benefits of K-One while working with your usual capture systems such as streptavidin, protein A/G, anti‑Fc, and anti‑Fab.
Rigorous quality process
Rely confidently on our quality control process, from bare gold nanoparticle manufacturing to K-One pre-functionalization and bioconjugation, for all scales of production.
You’ll first connect with our scientific sales team to discuss your needs and project goals.
You’ll meet our technical team to dive deep into your application and challenges. Based on these discussions, we build a detailed work plan and list all required materials, including your biomolecules (ligand(s), secondary antibody(ies), target(s), and medium (buffers or biological fluids)). It is important that we can work as close as we can from your application's final conditions, whatever the assay format.
Once we agree on scope, timing, and cost, we move straight to practical implementation.
Let’s Kick Things Off!
One week before work begins, we hold a comprehensive kick-off meeting where all details are finalized and final questions are answered. This is the moment to meet the person in charge of the project at Kimialys, who will be your contact throughout the entire project.
From there, you stay connected with our scientists at every step, keeping you fully informed on progress.
We deliver a comprehensive presentation and report on the bioconjugate optimization process and results. Based on our initial agreement, you receive your optimized bioconjuguates with buffers and detailed protocols — BonBecks included!
Curious about how our K-One gold bioconjugates can bind with your current needs?
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Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms
Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent
For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.
We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.
Gold-based sensor types:
For common SPR and SPRi platforms: Biacore, Horiba...
Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...
For chip-based diagnostic applications: electrochemical biosensors, QCM...
Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.
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