CRO services for advanced SPR analysis & assay development

Complete characterization through precise kinetics, reliable results, and simplified workflows.
We partner with leading biotech and pharmaceutical companies, as well as CROs and CDMOs, to deliver advanced biophysical analysis services, from molecular interaction screening to in-depth kinetic characterization.

K-One: unlocking new SPR possibilities.
Powered by K-One surface functionalization chemistry, we push the boundaries of SPR, enabling applications previously out of reach with conventional immobilization strategies. Our coating enhances SPR performance through precise ligand immobilization, ideal orientation, and complete protection of the surface. It ensures controlled surface density for accurate 1:1 Langmuir kinetics while minimizing non-specific binding and background noise.

We are a team of biosensor engineers and chemists with specialized mastery in SPR assay development.
At Kimialys, there is no single standardized protocol — each project requires its own assay design, tailored to the application and molecules at hand. From ligand immobilization strategy and optimization of K-One to instrument calibration, injection sequences and regeneration workflows, all parameters are carefully tuned to ensure assays that are robust, reproducible and adapted to complex biological systems.

A person holding a Lateral Flow Assay in his hand

5 reasons to work with Kimialys

1

Mastering complex systems

We enable even the most challenging SPR assays by customizing sensors and applying our K-One chemistry to fit your applications, molecules, and instruments.

2

Minimizing non-specific binding

With K‑One, the chip surface is optimally protected against non‑specific binding, ensuring minimal background noise and delivering consistently clean, reliable sensorgrams—even in complex samples.

3

Strong ligand attachment, high reproducibility

Ligands remain firmly attached while maintaining full activity on the surface, enabling repeated injection and regeneration cycles even under stringent washing conditions. This stability guarantees consistent results across complex samples such as plasma, serum, or supernatants.

4

Precise control of surface interactions

Our ability to adjust ligand density and control its homogeneous surface distribution allows us to work under affinity conditions by minimizing avidity effects and avoiding recapture. We can also work under avidity conditions when the project requires it.

5

Broad compatibility

Our K-One chips work with any gold-based biosensing platform, including Cytiva SPR, Horiba SPRi, Carterra HT-SPR and others. K-One surface chemistry enables direct ligand immobilization without intermediates or can integrate seamlessly with capture systems such as streptavidin, protein A/G, anti-FC and anti-Fab.

Kimialys SPR services: overview

Analytical SPR services

Binding interaction screening.

Kinetics characterization:

  • Affinity (KD)
  • Binding rate constants (kon, koff)
  • Avidity

Epitope binning and cross‑reactivity assessment.

Benefits

Drastic reduction of non-specific binding in buffer and complex samples.

Robust immobilization -Optimal reproducibility.

Enhanced signal-to-noise ratio- Increased sensitivity (up to 10x).

Scientific and strategic partnership – True assay development.

Our SPR instruments

Cytiva:

  • 2x T200 (SPR)
  • 1x T100 (SPR)

Horiba:

  • OpenPleX (SPRi)

Carterra (upcoming):

  • Ultra (HT-SPR)

Examples of complex molecules we handle

Antibodies:

  • mAbs/pAbs
  • Fc Receptors
  • Antibody fragments
  • Multispecific antibodies
  • Nanobodies
  • ADCs

Proteins:

  • C1q
  • IDPs
  • Membrane receptors
  • Coagulation factors
  • Lipid-bound proteins
  • Cytokines / growth factors
  • Peptides

Oligonucleotides:

  • ssDNA/dsDNA
  • RNA
  • Aptamers

Other entities:

  • Small molecules
  • Whole cells
  • Extracellular vesicles

Our working process

01

A warm welcome!

You’ll first connect with our sales team (Nicolas, Adrien or Cyril) to discuss your needs and project goals.

02

Technical dive & align

You’ll meet our technical team to dive deep into your application and challenges. Based on these discussions, we build a detailed work plan and list all required materials. Once we agree on scope, timing, and cost, we move straight to practical implementation.

03

Let’s kick things off!

One week before work begins, we hold a comprehensive kick-off meeting where all details are finalized and final questions are answered. This is the moment to meet the person in charge of the project at Kimialys, who will be your contact throughout the entire project. From there, you stay connected with our experimentalists at every step, keeping you fully informed on progress.

04

Your K-One chips are ready!

We deliver a comprehensive final report with all results and findings. Based on our initial agreement, you receive your optimized K-One biochips with buffers and detailed protocols — no chocolate included!

Reach out to us!

Curious about how our Surface Plasmon Resonance expertise can resonate with your current needs?

FAQ

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How do you optimize antibody pairing for LFA development?
  • Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications

  • Optimal ligand immobilization following Kimialys' proprietary protocol

  • In-house biomolecular interaction analysis with our own SPRi/SPR platforms

  • Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights

Binding interactions screening and epitope binning

We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.

We can immobilize any ligands:

  • Antibodies

  • Nanobodies

  • Small molecules

  • Proteins

  • DNA/RNA

  • Whole cells

  • Peptides

  • Aptamers

  • And more...

Our results are consistent and reliable for any complex biological matrices:

  • Plasma

  • Serum

  • Blood

  • Stem cell supernatant

  • Hybridoma supernatant

  • And more...

Kinetics characterization

Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.

Kinetics measured:

  • Affinity (KD)

  • Binding rate constants (kon, koff)

  • Avidity

How do you optimize antibody for LFA development?
  • Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications

  • Optimal ligand immobilization following Kimialys' proprietary protocol

  • In-house biomolecular interaction analysis with our own SPRi/SPR platforms

  • Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights

Binding interactions screening and epitope binning

We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.

We can immobilize any ligands:

  • Antibodies

  • Nanobodies

  • Small molecules

  • Proteins

  • DNA/RNA

  • Whole cells

  • Peptides

  • Aptamers

  • And more...

Our results are consistent and reliable for any complex biological matrices:

  • Plasma

  • Serum

  • Blood

  • Stem cell supernatant

  • Hybridoma supernatant

  • And more...

Kinetics characterization

Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.

Kinetics measured:

  • Affinity (KD)

  • Binding rate constants (kon, koff)

  • Avidity

How do you optimize antibody pairing for LFA development?
  • Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications

  • Optimal ligand immobilization following Kimialys' proprietary protocol

  • In-house biomolecular interaction analysis with our own SPRi/SPR platforms

  • Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights

Binding interactions screening and epitope binning

We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.

We can immobilize any ligands:

  • Antibodies

  • Nanobodies

  • Small molecules

  • Proteins

  • DNA/RNA

  • Whole cells

  • Peptides

  • Aptamers

  • And more...

Our results are consistent and reliable for any complex biological matrices:

  • Plasma

  • Serum

  • Blood

  • Stem cell supernatant

  • Hybridoma supernatant

  • And more...

Kinetics characterization

Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.

Kinetics measured:

  • Affinity (KD)

  • Binding rate constants (kon, koff)

  • Avidity

What factors impact the accuracy of an LFA test?
  • Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms

  • Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent

Our pre-functionalization technology is compatible with all gold-based sensors.

For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.

We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.

Gold-based sensor types:

  • For common SPR and SPRi platforms: Biacore, Horiba...

  • Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...

  • For chip-based diagnostic applications: electrochemical biosensors, QCM...

K-One® technology licensing

Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.

Still have a question ?

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Contact us