Complete characterization through precise kinetics, reliable results, and simplified workflows.
We partner with leading biotech and pharmaceutical companies, as well as CROs and CDMOs, to deliver advanced biophysical analysis services, from molecular interaction screening to in-depth kinetic characterization.
K-One: unlocking new SPR possibilities.
Powered by K-One surface functionalization chemistry, we push the boundaries of SPR, enabling applications previously out of reach with conventional sensorchips. Our coating enhances SPR performance through precise ligand immobilization, optimized orientation, and complete protection of the surface against non-specific interactions. It ensures controlled surface density for accurate 1:1 Langmuir kinetics while minimizing background noise.
We are a team of biophysists and chemists engineers with specialized mastery in SPR assay development.
At Kimialys, there is no single standardized protocol — each project requires its own assay design, tailored to the application and molecules at hand. From optimization of K-One and ligand immobilization strategy to instrument calibration, injection sequences and regeneration workflows, all parameters are carefully tuned to ensure assays that are robust, reproducible and adapted to complex biological systems.
Mastering complex systems
We enable even the most challenging SPR assays by customizing sensors and applying our K-One chemistry to fit your applications, molecules, and instruments.
Minimizing non-specific binding
With K‑One, the chip surface is optimally protected against non‑specific binding, ensuring minimal background noise and delivering consistently clean, reliable sensorgrams—even in complex samples.
Controlled ligand immobilization, high reproducibility
Direct covalent attachment of the ligands or via a capture approach while maintaining full activity on the surface, enabling repeated injection and regeneration cycles even under stringent conditions. This stability guarantees consistent results across complex samples such as plasma, serum, or supernatants.
Precise control of surface interactions
Our ability to adjust ligand density and control its homogeneous surface distribution allows working under affinity conditions by minimizing avidity effects or mass transport while avoiding recapture. We can also work under avidity conditions when the project requires it.
Broad compatibility
K-One surface chemistry enables direct ligand immobilization without intermediates or can integrate seamlessly with capture systems such as streptavidin, protein A/G, anti-Fc and anti-Fab antibodies.
Analytical SPR services
Binding interaction screening
Kinetics characterization:
Epitope binning and cross‑reactivity assessment
Our SPR instruments
Cytiva:
Horiba:
Benefits
Drastic reduction of non-specific binding in buffer and complex samples
Robust immobilization - Optimal reproducibility
Enhanced signal-to-noise ratio- Increased sensitivity (from 5-20x)
Scientific and strategic partnership – Complete assay development
Examples of complex molecules we handle
Antibodies:
Proteins:
Oligonucleotides:
Other entities:
You’ll first connect with our sales team (Nicolas, Adrien or Cyril) to discuss your needs and project goals.
You’ll meet our technical team to dive deep into your application and challenges. Based on these discussions, we build a detailed work plan and list all required materials.
Once we agree on scope, timing, and cost, we move straight to practical implementation.
One week before work begins, we hold a comprehensive kick-off meeting where all details are finalized and final questions are answered. This is the moment to meet the person in charge of the project at Kimialys, who will be your contact throughout the entire project.*
From there, you stay connected with our experimentalists at every step, keeping you fully informed on progress.
We deliver a comprehensive final report with all results and findings. We also provide raw data.
Curious about how our Surface Plasmon Resonance expertise can resonate with your current needs?
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Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms
Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent
For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.
We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.
Gold-based sensor types:
For common SPR and SPRi platforms: Biacore, Horiba...
Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...
For chip-based diagnostic applications: electrochemical biosensors, QCM...
Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.
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