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One for all, all for K-One

K-One is Kimialys’ proprietary technology platform, powering all our products and services.
This unique surface chemistry enables the optimal immobilization of any type of ligand (antibodies, proteins, oligonucleotides, small molecules, cells…) on any gold surface (nanoparticles, chips, electrodes), with applications spanning drug discovery and development: SPR analysis, bioanalysis, colorimetry, PCR workflows, immunogenicity testing, CDx development...

A self-assembled monolayer (SAM) offering full control over surface interactions.
K-One provides full control over ligand exposure, orientation, density, and distribution, maximizing binding site accessibility for target analytes and protecting the surface to minimize non-specific interactions, resulting in clear data without background noise.

Because achieving high sensitivity is meaningless if the data are not reliable.
Our functionalization technology enhances assays with unrivalled sensitivity, specificity, selectivity, reproducibility, and stability—without compromising one parameter for another—even in complex biological matrices such as blood, plasma, serum, or supernatants.

K-One is an enabling technology platform, designed by innovators for innovators.
It unlocks novel applications that were previously out of reach due to technological limitations, learn more below:

Diagram depicting ligand binding on gold nanoparticle or sensor chip surfaces. Figure 1 illustrates the scenario before K-One® application, with less structured binding, and Figure 2 shows the improved, organized binding post K-One® application.

Figure 1 - Ligands bind in an uncontrolled or even random fashion to the surface of gold nanoparticles or chips, resulting in only 20-30% of the ligands being available to interact with their target.
Figure 2 - The K-One® technology allows to control the ligands' exposure, density, and distribution, resulting in more than 90% of the ligands being available to interact with their target, while protecting the reaming part of the surface against undesired interactions.

FAQ

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How do you optimize antibody pairing for LFA development?
  • Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications

  • Optimal ligand immobilization following Kimialys' proprietary protocol

  • In-house biomolecular interaction analysis with our own SPRi/SPR platforms

  • Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights

Binding interactions screening and epitope binning

We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.

We can immobilize any ligands:

  • Antibodies

  • Nanobodies

  • Small molecules

  • Proteins

  • DNA/RNA

  • Whole cells

  • Peptides

  • Aptamers

  • And more...

Our results are consistent and reliable for any complex biological matrices:

  • Plasma

  • Serum

  • Blood

  • Stem cell supernatant

  • Hybridoma supernatant

  • And more...

Kinetics characterization

Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.

Kinetics measured:

  • Affinity (KD)

  • Binding rate constants (kon, koff)

  • Avidity

How do you optimize antibody for LFA development?
  • Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications

  • Optimal ligand immobilization following Kimialys' proprietary protocol

  • In-house biomolecular interaction analysis with our own SPRi/SPR platforms

  • Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights

Binding interactions screening and epitope binning

We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.

We can immobilize any ligands:

  • Antibodies

  • Nanobodies

  • Small molecules

  • Proteins

  • DNA/RNA

  • Whole cells

  • Peptides

  • Aptamers

  • And more...

Our results are consistent and reliable for any complex biological matrices:

  • Plasma

  • Serum

  • Blood

  • Stem cell supernatant

  • Hybridoma supernatant

  • And more...

Kinetics characterization

Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.

Kinetics measured:

  • Affinity (KD)

  • Binding rate constants (kon, koff)

  • Avidity

How do you optimize antibody pairing for LFA development?
  • Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications

  • Optimal ligand immobilization following Kimialys' proprietary protocol

  • In-house biomolecular interaction analysis with our own SPRi/SPR platforms

  • Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights

Binding interactions screening and epitope binning

We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.

We can immobilize any ligands:

  • Antibodies

  • Nanobodies

  • Small molecules

  • Proteins

  • DNA/RNA

  • Whole cells

  • Peptides

  • Aptamers

  • And more...

Our results are consistent and reliable for any complex biological matrices:

  • Plasma

  • Serum

  • Blood

  • Stem cell supernatant

  • Hybridoma supernatant

  • And more...

Kinetics characterization

Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.

Kinetics measured:

  • Affinity (KD)

  • Binding rate constants (kon, koff)

  • Avidity

What factors impact the accuracy of an LFA test?
  • Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms

  • Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent

Our pre-functionalization technology is compatible with all gold-based sensors.

For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.

We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.

Gold-based sensor types:

  • For common SPR and SPRi platforms: Biacore, Horiba...

  • Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...

  • For chip-based diagnostic applications: electrochemical biosensors, QCM...

K-One® technology licensing

Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.

Still have a question ?

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