Immobilize your ligands, not your research!
Our proprietary and patented K-One® surface chemistry is the result of over 10 years of academic research, led by Kimialys’ co-founder and Chief Scientific Officer, Dr. Claude Nogues, at Paris-Saclay University. K-One® unlocks applications that were previously out of reach due to technological limitations and sets a new standard for surface treatment in Life Science, especially for biomolecule detection and characterization.
In our context, ligands refer to biomolecules immobilized on biosensing surfaces such as nanoparticles, chips, or electrodes. These surfaces possess optical or electrical properties that enable the detection of a target once it is captured by the ligand.Without a properly optimized surface chemistry, ligand immobilization becomes uncontrolled, leading to random and variable attachment to the sensor surface. Mastering ligand immobilization is crucial, as it directly impacts key performance parameters such as sensitivity, specificity, and reproducibility in diagnostics and other in vitro assays.
Figure: conventional vs. K-One® -bases controlled ligand immobilization
Figure 1 - Ligands bind in an uncontrolled or even random fashion to the surface of gold nanoparticles or chips, resulting in only 20-30% of the ligands being available to interact with their target.
Figure 2 - The K-One® technology allows to control the ligands' exposure, density, and distribution, resulting in more than 90% of the ligands being available to interact with their target, while protecting the reaming part of the surface against undesired interactions.
In a nutshell, this is what K-One® brings to the table:
P.S. K-One® has been successfully implemented on gold surfaces. If you're interested in applying our surface chemistry to another material, we’d be glad to explore a co-development to adapt and optimize the chemistry for that material.
P.P.S. Our K-One® surface chemistry is also available for licensing, offering the opportunity to integrate it directly into your production workflows and quality standards.
You know the basics about K-One®, it’s time to explore our solutions!
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Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of SPR sensor chips using K-One® surface chemistry, specifically customized to your applications
Optimal ligand immobilization following Kimialys' proprietary protocol
In-house biomolecular interaction analysis with our own SPRi/SPR platforms
Reliable access to binding and kinetics data, complemented by high-quality reports and presentations, offering actionable insights
We harness both our expertise in SPRi and proprietary K-One® technology on chip to swiftly identify and rank both desired and undesired binding interactions among numerous candidates. This ensures our customers receive consistent and reliable results, accelerating their pursuit of new therapeutics.
We can immobilize any ligands:
Antibodies
Nanobodies
Small molecules
Proteins
DNA/RNA
Whole cells
Peptides
Aptamers
And more...
Our results are consistent and reliable for any complex biological matrices:
Plasma
Serum
Blood
Stem cell supernatant
Hybridoma supernatant
And more...
Reliable measurement of kinetic constants is pivotal for the development of new therapeutics. Leveraging our proprietary K-One® technology, we stand out in the SPR landscape by delivering precise characterization analysis for all biologics and compounds.
Kinetics measured:
Affinity (KD)
Binding rate constants (kon, koff)
Avidity
Pre-functionalization of sensor chips using K-One® surface chemistry, specifically customized to your applications and analytical platforms
Supply of batches of pre-functionalized sensor chips + ligand immobilization protocol and reagent
For companies looking to manage their own analyses, yet eager to leverage our expertise in chip functionalization and our proficiency in tackling complex analytical challenges.
We provide custom pre-functionalized sensor chips, specifically designed for your ligands, analytical platform, and applications, ensuring that your analyses run smoothly and effectively.
Gold-based sensor types:
For common SPR and SPRi platforms: Biacore, Horiba...
Other SPR-based biosensors: LSPR, Fiber Optic SPR, LR-SPR...
For chip-based diagnostic applications: electrochemical biosensors, QCM...
Our K-One® surface chemistry technology is available for licensing, offering the opportunity to integrate it within your own production environment and standards.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique.
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